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| Registros recuperados: 978 | |
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| Rodríguez,Paula; Sierra,Wilson; Rodríguez,Sonia; Menéndez,Pilar. |
| The forest industry in Uruguay has grown considerably during the last decade. Eucalyptus plantations account for 74% of the forested land, with Eucalyptus globulus being the most widely distributed species. This industry is dedicated exclusively to the production of wood without exploiting the by-products (leaves and small branches). Eucalyptus leaves are known to contain important amounts of essential oils composed primarily of 1,8-cineole (1,3,3-trymethyl-2-oxabicyclo[2.2.2]octane). In this work, the biotransformation of 1,8-cineole, is achieved using a native bacterium (Rhodococcus sp.) which was isolated from the soil of Eucalyptus forest. A 98% of bioconversion was achieved. Three different optically pure compounds were obtained, and they were... |
| Tipo: Journal article |
Palavras-chave: Biocatalysis; Monoterpenes; Eucalyptus; Green Chemistry. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300011 |
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| Islam,Aminul; Ghosh,Runa; Banerjee,Dipankar; Nath,Piali; Mazumder,Upal Kanti; Ghosal,Shibnath. |
| “Shilajit” is a panacea in Ayurveda, the Indian traditional system of medicine. The major bioactives of “shilajit” have been identified as dibenzo-α-pyrones (DBPs), its oligomers and aminoacyl conjugated derivatives. These bioactive compounds play a crucial role in energy metabolism in all animal cells including those of man. 3-hydroxydibenzo-α-pyrone (3-OH-DBP), a key DBP component of “shilajit” is converted, among other products, to another active DBP derivative, viz. 3,8-hydroxydibenzo-α-pyrone, 3,8(OH)2-DBP, in vivo, when its precursor is ingested. 3,8(OH)2-DBP is then involved in energy synthesis in the mitochondria in the reduction and stabilization of coenzyme Q10... |
| Tipo: Journal article |
Palavras-chave: Coenzyme Q10 reduction; Electron transport chain humification; Paleohumus. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300001 |
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| Velankar,Harshad Ravindra; Heble,Mohan Ram. |
| This paper reports biotransformation of (L)-citronellal to (L)-citronellol using free and immobilized cells of Rhodotorula minuta. The culture preparation variables such as pH, temperature and incubation period for obtaining maximum cell growth of R. minuta were optimized. The optimized culture conditions for free and immobilized cells of R. minuta. have been compared for (L)-citronellal biotransformation. The various factors such as the optimum substrate concentration and the time of substrate addition at varying cell concentrations during the growth of yeast culture were also studied. Highest (L)-citronellol concentration of 3.5 gl-1 was obtained with free cell catalyzed biotransformation at pH 5.5, 27ºC and 150 rpm after 8 hrs using initial... |
| Tipo: Journal article |
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| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582003000200004 |
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| Zhao,Ming; Zhou,Jin yan; Li,Zhi dong; Song,Wei wei; Tan,You jiu; Tan,Hong. |
| Botrytis cinerea is a necrotrophic pathogen causing pre- and post-harvest diseases in at least 235 plant species. It manifests extraordinary genotype and phenotype variation. One of the causes of this variation is transposable elements. Two transposable elements have been discovered in this fungus, the retrotransposon (Boty), and the transposon (Flipper). In this work, two complete (Boty-II-76 and Boty-II-103) and two partial (Boty-II-95 and Boty-II-141) long terminal repeat (LTR) retrotransposons were identified by an in silico genomic sequence analysis. Boty-II-76 and Boty-II-103 contain 6439 bp nucleotides with a pair of LTRs at both ends, and an internal deduced pol gene encoding a polyprotein with reverse transcriptase and DDE integrase domains. They... |
| Tipo: Journal article |
Palavras-chave: Botrytis cinerea; LTR retrotransposons; Molecular marker; Plant pathogen; Population diversity; Transposable elements. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300002 |
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| Cao,Yuanyuan; Yao,Jianming; Li,Jun; Chen,Xiangsong; Wu,Jinyong. |
| To obtain an industrial strain with high lipid yield, the wild strain G0 of Geotrichum robustum was mutated by means of nitrogen ions implantation. Mutagenic effects of strain G0 by low energy N+ ion implantation were studied. The experimental results indicated that the survival rate curve took a ?saddle? shape, and the positive mutation rate was increased to 22.00% at the dose of nitrogen ions 2.0 x 10(15) ions/cm² when the survival rate was 28.60%. By repeated screening, a high lipid producing strain G9 was obtained. The biomass, lipid content and lipid yield of the mutant can reach 40.25 g/L, 71.14% and 28.63 g/L after cultured in a 5L fermenter for 8 days, increasing by 52.81%, 68.82% and 157.93% to those of wild strain, respectively. Analysis results... |
| Tipo: Journal article |
Palavras-chave: Fatty acids; Geotrichum robustum; High lipid producing strain; Ion beam implantation. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600002 |
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| Xu,Ji-Fei; Mi,Yuan-Ting; Ren,Nan-Qi. |
| The buffering effect of acetate on hydrogen production during glucose fermentation by Ethanoligenens harbinense B49 was investigated compared to phosphate, a widely used fermentative hydrogen production buffer. Specific concentrations of sodium acetate or phosphate were added to batch cultures, and the effects on hydrogen production were comparatively analyzed using a modified Gompertz model. Adding 50 mM acetate or phosphate suppressed the hydrogen production peak and slightly extended the lag phase. However, the overall hydrogen yields were 113.5 and 108.5 mmol/L, respectively, and the final pH was effectively controlled. Acetate buffered against hydrogen production more effectively than did phosphate, promoting cell growth and preventing decreased pH.... |
| Tipo: Journal article |
Palavras-chave: Acetate; Biohydrogen; Buffering action; Ethanoligenens harbinense phosphate. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000500002 |
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| Benítez-García,Israel; Vanegas-Espinoza,Pablo Emilio; Meléndez-Martínez,Antonio J; Heredia,Francisco J; Paredes-López,Octavio; Del Villar-Martínez,Alma Angélica. |
| Background The properties of natural pigments, such as antioxidants, functional, medical, and nutraceutical, have demonstrated the advantages of these natural compounds over synthetic ones. Some products are accepted only when they are pigmented with natural, food-quality colorants: for example poultry products (manly marigold flower extracts). Carotenoids such as β-carotene, β-criptoxanthin and lutein are very attractive as natural food colorants due to their antioxidant and pro-vitamin activities which provide additional value to the target products. Marigold (Tagetes erecta) is an Asteraceous ornamental plant native to Mexico, and it is also important as a carotenoid source for industrial and medicinal purposes but nowadays its... |
| Tipo: Journal article |
Palavras-chave: 2 4-D; BA Cell development Lutein Marigold. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000300001 |
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| Cimino,Cecilia; Vairo Cavalli,Sandra; Spina,Francisco; Natalucci,Claudia; Priolo,Nora. |
| The objective of this work was the optimization of the conditions of in vitro culture for callus production of Silybum marianum (L.) Gaertn. (Asteraceae). Sections of cotyledons, previously disinfected by washing successively with ethanol 70º, NaClO (10% w/v) and Tween 20 (0.05% v/v) and rinsing with sterile distilled water, were used as explants. For its initial culture, B5 medium supplemented with BA and 2,4-D solidified with phytagel was used, and a 63% survival was achieved. To obtain callus, two solid media were assayed (S1 and S2) using B5 medium supplemented with growth regulators (BA and 2,4-D or NAA and BA, respectively). The calli were grown at 25ºC during 45 days in darkness. Growth kinetics was studied using S1 medium... |
| Tipo: Journal article |
Palavras-chave: Asteraceae; Callus; Rhizogenesis; Silybum marianum. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300012 |
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| Erisen,Semiha; Yorgancilar,Mustafa; Atalay,Emine; Babaoglu,Mehmet; Duran,Ahmet. |
| A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [?-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was... |
| Tipo: Journal article |
Palavras-chave: Astragalus; Callus; Organogenesis; Plant regeneration. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600013 |
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| Tolmachov,Oleg. |
| Many gene therapy strategies require transfer of high-molecular weight DNA into human cells. To enable clinical trials, these vectors need to be produced on a large scale and at low cost. The production of effective high-capacity vectors like HSV-amplicons and helper-dependent adenoviral vectors is difficult to up-scale, so new inexpensive vectors are needed for the efficient delivery of high-molecular weight DNA to human cells. Bacteriophage lambda vectors can accommodate up to about 46 kb of therapeutic DNA and can be easily produced in an industrial setting. However, the lambda vectors transfer DNA into mammalian cells with only a low efficiency. It was shown that bacteriophage lambda virions ejected their DNA in the presence of the purified receptor... |
| Tipo: Journal article |
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| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400012 |
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| Petrova,Ventsislava Yankova; Rasheva,Tanya Vassileva; Kujumdzieva,Anna V.. |
| Catalase and superoxide dismutase activities have been explored in the yeast Saccharomyces cerevisiae during batchwise growth experiment. During the diauxic growth in YPD medium high Ys values were obtained (0.415 - 0.423) and correlation between the total activities of both enzymes has been found. A mitochondrial fraction from three type strains of Saccharomyces cerevisiae has been isolated. The purity of this fraction was proved through different enzyme assays: hexokinase, glucose-6-phosphate dehydrogenase, D-amino acid oxidase, isocitric lyase, succinate dehydrogenase. Then the catalase, peroxidase, Mn and Cu/Zn superoxide dismutase activities were evaluated in the mitochondrial fraction. Polyacrylamide gel electrophoresis separations allowed to... |
| Tipo: Journal article |
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| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000100010 |
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| Registros recuperados: 978 | |
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